Page 102 - Raw Diet References Book 2019

1.2.3 Food

Food type, flavour, and production codes of food sufficient to feed all animals for the duration of
representing the composite feed shall be recorded. the pre-collection and collection periods. If chromic
The food source shall remain constant throughout oxide is used, approximately 0.25% of a high quality
the test period. chromium III oxide (Cr203) free of soluble chromium
shall be mixed with the food.
The indicator shall be uniformly mixed in a quantity

1.2.4. Food allowances

The amount of food presented to each animal may daily maintenance energy requirements [110kcal
be based upon existing data on the quantity of food (460kJ) ME per kg BW0.75 for dogs and about 60kcal
required to maintain body weight, or the estimated (250kJ) ME per kg BW for cats] (See ANNEX 2 - Energy).

1.2.5 Times of feeding

Animals shall be fed at least once daily and at the instructions for the product. The excess food shall be
same time each day. Water shall be available at all weighed back after feeding.
times. Food shall be fed as is, or per normal feeding

1.2.6 Pre-trial termination

If, during the pre-collection phase, the food consumption by a majority of the animals, the trial
is continually rejected or results in minimal shall not proceed into the collection phase.

1.2.7 Collection

Faeces collection It is imperative that all collection collection. Aliquots of faeces from five separate days
containers be clearly marked using double labels shall be collected. Every effort should be made to avoid
or any alternative adequate coding. The labels shall collecting contaminants such as hair. The aliquots shall
include the animal number, diet number, and dates of be dried and pooled per individual animal.

Urine collection During the collection period, all metabolizable energy. Every effort should be made to
daily urine shall be collected for each animal and avoid collecting contaminants such as hair.
weighed, unless a correction factor is used to estimate

1.2.8 Sample preparation

Food The food shall be blended to ensure a uniform sample should be frozen and retained until assay
consistency and an adequate quantity used for results have been reviewed and found acceptable.
appropriate assays. Ample quantities of the remaining

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