Page 104 - Raw Diet References Book 2019

If urine is not collected ME (kcal or kJ/g) = DE - (DP x correction factor for energy lost in urine)

Correction factor for energy lost in urine (Kienzle 1.25kcal or 5.23kJ/g for dogs
et al. 1998): 0.86kcal or 3.60kJ/g for cats

2. QUANTITATIVE COLLECTION METHOD

2.1 Introduction

This feeding protocol has been designed in order to dog and cat food metabolizable energy protocols –
determine ME of cat & dog foods in a way not harmful Quantitative Collection Method” (AAFCO 2007).
for cats and dogs and is adapted from the “AAFCO

2.2 Protocol

2.2.1 Animals

A minimum of six fully grown animals at least one breed. Animals shall be individually housed during
year of age shall complete the test. The animals shall the trial (collection period).
be in good health and of known weight, sex and

2.2.2 Feeding procedures

Feeding procedures shall be standardized. The The second phase shall be the total collection
feeding shall consist of two phases. period of at least four days (96 hours) for dogs and
five days (120 hours) for cats.
The first phase shall be the pre-collection period
of at least three days for dogs and five days for cats The amount of food offered during the second
(Nott et al. 1994) with the objective of acclimatising phase shall remain constant. Food intake shall be
the test animals to the diet and adjusting food intake, recorded throughout both phases.
as necessary, to maintain body weight.

2.2.3 Food

Food type, flavour, and production codes The food source shall remain constant throughout
representing the composite feed shall be recorded. the test period.

2.2.4 Food allowances

The amount of food presented to each animal may maintenance energy requirements [110kcal (460-480kJ)
be based upon existing data on the quantity of food ME per kg BW0.75 for dogs and about 60kcal (250-293kJ)
required to maintain body weight or the estimated daily ME per kg BW for cats] (See ANNEX 2 - Energy).

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